For many scientific researches, mostly in
medical, chemical, biology, organic and biochemistry, the transillumination is
used for experiment. Transillumination is the process of imaging out a sample
by illuminating, also known as light transmission goes through the samples and
create sample image. Transilluminators
are the tools that used for this process. Among well-known and widely used
processes, the Blue Light transilluminators
and UV variant are notable but the first one is mostly preferred because of
few reasons. First, UV oriented tool is not safe as it produces and spreads UV
ray that may cause harm to the sample, DNA samples are more vulnerable. Second,
it is harmful for the human body, as it is reported that, UV ray cause skin
diseases to human body and excessive radiation may lead to skin cancer. These
reasons give Blue Light variant preferences
over the UV device. These blue light tools don't cause any harm to human body,
don't burn the body and irritate eyes. And, also keep the damage at lowest to
the samples. But, scientific researchers are too much sensitive and also
concerns many other security facts.
Moreover, there are wide varieties of vendors
who market blue light transilluminators. So, there are few
certain facts should be considered when choosing a transilluminator. Make sure that your device has been featured
following the standard for safety of European community. Be sure about your
required products dimension and get informed about your chosen one. The dimension
of the viewing surface is also important as you will experiment basically on
this. Know about detail product specification, for instance get informed about
LED life span, as expected life span is 50,000 hours. Most of the experiments
are done with gel; say for example, for DNA synthesis, gel is necessary. For,
the synthesis the excitement of the gel is also necessary. Make sure that your device
handles the excitement of gel properly. An adapter is essential for the
effective gel documentation system, to get better result, make sure that your
device got electric power adapter. Be sure about the versatility of your
device, the more dyes it can illuminate, it is considered better transilluminator. Your device should at
least cover Ethidium Bromide, SYPRO Ruby, GetGreen, SYBRSafe, GelRed, EtBr
since they are standard and widely used. For better result, the S/N ratio
should be at least. The standard wavelength is 470 nm; make sure your device
features the same.
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