About Blue Light Transillumination

Transillumination is a scientific experimental process, mostly used in biology, biochemistry and medical laboratory to experiment samples by illuminating that is light transmitting through the samples. The final goal of transillumination is to produce an image of the sample to be experimented. Different transilluminators are used to accomplish transillumination process, among which Blue light transilluminators and UV variation are well known and widely used. Although, both tools are used by the first one is mostly preferred. This is because, the UV transilluminators are reported that, these generate ultraviolet ray that causes damage to the sample, and also serious harm to human body. For instances, UV variant may burn your skin and eyes. This also may damage DNA samples and reduce cloning efficiency. Different vendors provide Blue light transilluminators with different functionalities, dimensions and properties. So, while choosing this, few points needed to be considered. As, most of the experiments employ gel, get informed that how does your chosen machine handle these gels, be sure that your devices fulfill the safety prerequisites of European community. Get informed about the product dimension and choose the perfect one. The viewing surface dimension is also needed to be checked. While choosing, notice the LED life span, for example expected life span is 50,000 hours. Your machine should provide necessary excitement for the gel. It should be optimized to use with different samples, nucleic acids, protein stains etc. For easy and effective inside gel documentation system, there should be an electrical power corded adapter. The better result can be yielded if the light is brighter and the light emission is uniform. So, make sure that these features are standard on your device. 

The more number of ranges of dyes it can illuminate, the better the transilluminator it is. So, make sure that you device can illuminate at least the standard number of dyes. It should illuminate at least Ethidium bromide, SYPRO Ruby, GetGreen, SYBRSafe, GelRed, EtBr as they are standard and widely used. The S/N ratio is very important while imaging, make sure that your device keep this ratio at lowest. Consider the wavelength; expected wavelength should be around 470 nm.  A number of vendors market these devices at different price levels based on included features and functionalities from $200 to $1000. The price depends on the device dimensions, experiment surface dimensions, wavelength etc. And, not all the devices are suitable for all experiments; grab the perfect product for perfect lab-work.